P2X7 (EXTRACELL DOMAIN); RBX-200UL

Analysis Note

ControlIncluded free of charge with the antibody is 40 µg of control antigen (lyophilized powder). For negative control, preincubate 1 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Western blotting: 1:200 using ECL on rat brain membranes.

Flow cytometry, indirect: 5-10 µg antibody per 1x10E6 cells.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Anti-P2X7 Receptor Antibody, extracellular domain is an antibody against P2X7 Receptor for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: extracellular domain

Highly purified peptide corresponding to residues 136-152 of mouse P2X7 (Accession Q9Z1M0).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Specificity

Recognizes P2X7, extracellular domain.

Storage and Stability

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.